Figure 6.

Involvement of Multiple WRKY Transcription Factors in INF1-PTI and AVR3a-ETI ROS Bursts.

(A) Enhancement of flg22-triggered ROS burst in MAPK- or WRKY-silenced plants. Flg22-triggered ROS bursts were measured for 60 min in leaves silencing SIPK/WIPK (S/W), WRKY7, 8, 9, and 11 (7/8/9/11), or RBOHB (NbB). Total photon counts of each treatment during 60 min were graphed in the right panel.

(B) Effects of multiple WRKY gene silencing on effector-triggered ROS burst. Silenced leaves were coinoculated with Agrobacterium carrying pER8:AVR3a-HA and pBinPlus:R3a and then were injected with 20 μM estradiol 24 h later. To detect ROS generation, inoculation sites were infiltrated with 0.5 mM L-012 solution 24 h after estradiol injection and were monitored using a CCD camera. Chemiluminescence intensities were quantified by a program equipped with a photon image processor.

(C) Effects of multiple WRKY gene silencing on INF1-triggered ROS burst. INF1-ROS burst was detected in silenced leaves 24 h after agroinfiltration.

Asterisks indicate statistically significant differences compared with TRV (t test, *P < 0.05 and **P < 0.01). Data are means ± se from at least five experiments.