Figure 8.

Involvement of Multiple WRKY Transcription Factors in RBOHB-Dependent ROS Burst.

(A) ROS generation by overexpression of WRKY transcription factors. Leaves were inoculated with Agrobacterium carrying the indicated gene expression constructs. ROS generation was detected 24 h after agroinfiltration as described in Figure 6B.

(B) WRKY-induced ROS generation via RBOHB. TRV control, RBOHB (NbB)-, and BAK1-silenced leaves were inoculated with Agrobacterium, and ROS were measured 24 h after agroinfiltration.

(C) ROS generation by overexpression of phospho-mimicking mutants.

(D) Detection of phospho-mimicking mutants by anti-HA antibody. C, control plant; W, wild-type WRKY11; D, phospho-mimicking mutant; A, nonphosphorylated mutant.

Letters represent each significance group, determined through Tukey's multiple range test. Asterisks indicate statistically significant differences compared with TRV ([B] and [C]) (t test, **P < 0.01). Data are means ± se from at least three experiments.