Figure 8.

Purification and Filter Binding Assays of Defined Motifs of rHCF145 Protein.

(A) Release of the entire recombinant HCF145 protein, a fragment containing the SRPBCC domains, as well as the tandem repeated TMR motifs from the MBP fusion.

(B) Filter binding assays of purified proteins shown in (A) using increasing concentrations of protein up to 200 nM for the full-length protein and up to 2000 nM for the truncated versions. B, bound RNA; U, unbound RNA.

(C) Quantitative determination of the RNA fraction bound to HCF145 to evaluate the dissociation constant (K_d) value.