Figure 5.

Structural Variations of the Kala4 Promoters in 21 Black Rice Varieties.

The Kala4 promoters of 21 black rice varieties were classified into three types based on the length of inserted fragments. Type I promoters have the longest insertion (11.02 kb) with the same size as ‘Hong Xie Nuo.’ Type II promoters have a deletion of ∼2.7 kb at the 5′ junction. Type III promoters have the 2.7-kb deletion at the 5′ junction and a 2.4-kb deletion at the 3′ junction. The original Kala4 ORF sequence (blue region), insertion from ∼83 kb upstream of Kala4 (orange), and the duplicated segment of Kala4 (red) for each type are shown.