Table 1. General GUS Expression Patterns in Transgenic Lines from Predicted Enhancers.
| Predicted Enhancer (Short Name) | Size (bp) | Tissues with GUS Expression |
||
|---|---|---|---|---|
| Root (F/R)a | Leaf (F/R) | Flower (F/R) | ||
| Common DHS 1 (C1)b | 568 | −/− | +++/+++ | +++/+ |
| Common DHS 2 (C2)b | 684 | −/− | +/+ | +++/− |
| Common DHS 3 (C3)b | 393 | −/NA | −/NA | −/NA |
| Common DHS 4 (C4) | 580 | −/− | +++/+++ | +++/− |
| Common DHS 5 (C5) | 580 | −/++ | −/− | +++/− |
| Common DHS 6 (C6) | 384 | −/NA | −/NA | −/NA |
| Common DHS 7 (C7) | 403 | −/NA | −/NA | −/NA |
| Common DHS 8 (C8) | 562 | −/− | +/+++ | −/− |
| Leaf DHS 1 (L1) | 588 | −/− | +/++ | −/NA |
| Leaf DHS 2 (L2) | 444 | +/+ | +/+ | −/NA |
| Leaf DHS 3 (L3) | 625 | +++/+++ | +/+++ | −/NA |
| Flower DHS 1 (F1) | 622 | −/− | +++/+++ | +++/+++ |
| Flower DHS 2 (F2) | 533 | −/− | −/− | +/++ |
| Flower DHS 3 (F3) | 806 | −/NA | −/NA | −/NA |
a
Forward/reverse direction of each predicted enhancer within the report construct. +++, More than 75% positive transgenic plants or 50 to 75% positive transgenic plants with consistently strong signals in the same tissue(s); ++, 50 to 75% positive transgenic plants with consistently weak signals in the same tissue(s); +, <50% positive transgenic plants with consistently strong signals in the same tissue(s); −, <50% positive transgenic plants with inconsistent signals in different tissue(s) or no signals in any tissues; NA, not available.
b
C1, C2, and C3 are three putative enhancers identified previously by the enhancer trapping method (Michael and McClung, 2003).