Figure 3.

Co BUB2 and Co BFA1 Regulate G1/S Progression in C. orbiculare.

(A) Schematic representation of the C. orbiculare strain expressing GFP-LacI-NLS, which associates with the LacO array integrated into C. orbiculare chromosomes.

(B) Mean percentage (±se; n = 3) of wild-type (WT) and Co bub2Δ cells with three patterns of GFP-LacI spots. Scoring: single fluorescent spot at one nucleus, putative G1 phase (black); closely spaced double spots at one nucleus, putative post-S phase or G2 phase (red); and single spots at both nuclei, putative post-M phase (blue). At least 100 conidia were scored at each time point.

(C) Representative images of LacO/LacI-GFP introduced wild type and Co bub2Δ during appressorium development. Enlarged images of the nucleus with one or two LacI-GFP spots are shown in the boxed areas. Arrowheads indicate interphase nuclei. Nuclear staining was performed using Hoechst 33342. Bars in DIC panels = 5 μm; bars in enlarged image = 1 μm.