Figure 5.

Co Tem1 Interacts with Co Bub2 and Co Bfa1 in Yeast Two-Hybrid Assays.

(A) Schematic representation of C. orbiculare Tem1 and S. cerevisiae Tem1p. The 95-amino acid sequence of the N-terminal end of Co Tem1 is specific to filamentous fungi and missing in yeast. Rab family domains of Co Tem1 and Tem1p are labeled in gray.

(B) Interaction of Co Tem1 with Co Bub2 or Co Bfa1 and interaction of S. cerevisiae Tem1p with Co Bub2 or Co Bfa1, as determined by a yeast two-hybrid experiment. Interaction was assessed from yeast growth on SD media lacking –Trp –Leu (DDO), –Trp –Leu + AbA (DDO/A), –Trp –Leu –Ade –His (QDO), and –Trp –Leu + AbA + X-α-Gal (DDO/A/X). The empty-AD vector was used as a negative control. +, Interaction; –, no interaction.