Figure 6.

Introduction of Co Tem1^T128N Restored the Wild-Type Nuclear Behavior in Co bub2Δ and Co bfa1Δ.

(A) Schematic representation of the Co Tem1^T128N allele, which was introduced into wild-type C. orbiculare and Co bub2Δ and Co bfa1Δ mutants.

(B) Representative images of nuclear distribution during appressorium morphogenesis in the Co Tem1^T128N-introduced strain. Pregermination conidia and appressorium-forming conidia were fixed and nuclei stained with DAPI and calcofluor white at 3 and 10 hpi, respectively. Bar = 5 μm.

(C) Mean percentage (±se; n = 3) of cells with various patterns of nuclear distribution for the wild type, Co bub2Δ, and Co bfa1Δ, with and without the introduced Co Tem1^T128N isoform. Lower left panel indicates conidia with two or three nuclei and an aberrant extra septum in the middle. For other scoring, see legend to Figure 1. At least 200 conidia were scored at each time point.