Figure 4.

Expression of Root Development and Auxin/Cytokinin Response Genes in Crown Roots of ERF3 Transgenic Plants.

(A) Relative expression level of root developmental genes determined by RT-qPCR.

(B) Relative expression level of four rice Aux/IAA genes and four type-A RR genes determined by RT-qPCR.

The PCR signals in (A) and (B) were normalized with ACTIN1 transcripts. Transcript level from the wild type was set at 1. ACTIN1 was used as an internal control. Data represent the means ± sd of three independent biological replicates.

(C) In situ hybridization detection of RR2 transcripts in the crown root tip and crown root primordia in the wild type (WT), ERF3 overexpression (OE3), and ERF3 amiRNAs (amiE3) with an antisense or sense probe (control). Arrows indicate crown root initials. Bars= 75 μm.