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. 2015 Aug 25;27(9):2469–2483. doi: 10.1105/tpc.15.00227

Figure 7.

Figure 7.

Genetic Relationship between WOX11 and ERF3 in Controlling Rice Crown Root Development and RR2 Expression.

(A) Transcript levels of ERF3 and WOX11 in roots of wild-type (WT) and amiERF3/wox11 plants. The PCR signals were normalized with ACTIN1 transcripts. Transcript level from the wild type was set at 1. Bars are means ± sd from three technical replicates.

(B) to (D) Crown root phenotypes of indicated genotypes. OxWOX11, WOX11 overexpression lines; OxERF3, ERF3 overexpression lines; ERF3:ERF3/wox11, ERF3 cDNA under the ERF3 promoter in the wox11 background. Significant differences between samples (t test) are indicated by different letters. Bar = 1 cm in (B) and 5 cm in (C).

(E) RR2 transcripts detected by in situ hybridization in the wild type, wox11, ERF3:ERF3/wox11, and amiERF3/wox11. Arrows indicate crown root initials. Bar = 75 μm.