Table 1.
Purification profile of the linear and cyclized lichenase
| Linear lichenase | Cyclized lichenase | |||
|---|---|---|---|---|
| Before ITC | After ITC | Before ITC | After ITC | |
| Activity (U) | 8620.88 | 1911.94 | 3768.75 | 638.70 |
| Protein (mg) | 303.98 | 15.08 | 339.3 | 11.05 |
| Specific activity (U/mg) | 28.36 | 126.78 | 11.10 | 57.73 |
| specific productivity (U/g)a | 3533.15 | 783.58 | 1544.57 | 261.76 |
| Activity recovry (%) | 22.18 | 16.95 | ||
| Purification fold | 4.47 | 5.20 | ||
The table data were based on 1L culture
a The specific productivity means the activity productivity of dry cell weight (1 L culture)