Fig. 4.

Amino acid mutation in the H2-D^b-NP₃₆₆ epitope and CD8+ T cell recognition following virus infection in AAD mice. a Sequence electropherograms showing the change from Asparagine (N) to Glutamine (Q) at position 370 in the H2-D^b-NP₃₆₆ epitope of the PR8-NP_N370Q virus. b Mice were infected i.n. with 10^2.4 TCID₅₀ of PR8 or PR8-NP_N370Q virus. Nine days later, the mice were sacrificed and the frequency of H2-D^b-NP₃₆₆-specific IFN-γ-producing T cells was measured by using an ELISPOT assay on spleen-derived lymphocytes from individual mice. Results are expressed as means of triplicate wells ± SD. The stained wells of representative samples are shown below the graphical representations of the number of spot forming units (SFU) per 10⁶ cells