Fig. 2.

Mock clinical LODs for the PTV and PICV assays. Total RNA extracted from 10-fold serial dilutions of (a) PTV and (b) PICV spiked into water, sera, and whole blood were extracted in triplicate and tested with the final PTV and PICV assay listed in Table 1. In each assay, replicates that had no amplification curves or Cq values falling above 40 were given a base value of 40. Error bars represent the standard deviation of three replicates