Figure 2.

Expression analysis of AtHD2D and of AtHD2D-gfp in transgenic Arabidopsis thaliana plants. (A) Diagram of the 35S:AtHD2C-gfp construct. AtHD2D CDS was fused with the gfp-coding region driven by a 35S promoter. The primers, pr1, pr2, and pr3, used to analyze AtHD2D and AtHD2D-gfp in transgenic plants were shown. (B) PCR analysis of 35S:AtHD2D-gfp transgenic and wild-type (WT) plants using primer pairs of pr1 and pr3. No PCR product was found in WT. (C) RT-PCR analysis of AtHD2D and AtHD2D-gfp expression in transgenic and wild-type plants using the primer pairs of pr1 and pr2 or pr1 and pr3. No RT-PCR product of AtHD2D-gfp was found in WT.