Table 1.

Sample names, corresponding SRA/ENA identifiers, number of reads, read length, number of bases, and the average fragment length for the samples used for evaluation of the EAGER pipeline

Sample	SRA ID/ENA ID	# of reads	Read length	# of bases	Avg. fragment length
3077	SRX275526	6,029,646	76	916,506,192	60.87
Refshale16	SRX276068	39,915,365	76	6,067,135,480	79.91
Jorgen625	SRX275549	15,101,591	200	6,040,636,400	164.24
SK2	SRX275535	54,243,849	100	10,848,769,800	62.84
SK8	SRX275538	9,898,159	76	1,504,520,168	81.41
LBK1	SAMEA2697125	227,266,922	101	45,907,918,244	69.71
LBK2	SAMEA2697125	222,751,961	101	44,995,896,122	69.69
LBK3	SAMEA2697125	227,779,612	101	46,011,481,624	69.72
LBK4	SAMEA2697125	207,406,901	101	41,896,194,002	69.72
LBK5	SAMEA2697125	207,983,311	101	42,012,628,822	69.67
LBK6	SAMEA2697125	208,835,520	101	42,184,775,040	69.71
LBK7	SAMEA2697125	213,784,583	101	43,184,485,766	69.68
LBK8	SAMEA2697125	228,184,096	101	46,093,187,392	69.71

For the LBK data set, we chose to evaluate a single lane of data (LBK1), as the other samples (LBK2–8) showed very similar features