Figure 2.

Transformation rescue experiments of the RNase T‐knockout E. coli K12 strains (Δrnt) by expression of RNase T mutants. The RNase T deletion strain (K12‐Δrnt) had a slow‐growth phenotype that can be fully rescued by introducing a plasmid expressing wild‐type RNase T as shown by the colony size variations of E. coli K12 strains on LB plates. The negative control of the “Binding” mutant (F29A/E73A/F77A) could not rescue the slow growth phenotype. The 2W1A and 2Y1A mutants moderately rescued, whereas the 4W, 4W1A, 2W3A, 4Y, 4Y1A, and 2Y3A mutants could not rescue, the slow growth phenotype of the RNase T deletion strain.