Table 1.
Endpoint Activity for Selected Peptides with KDAC8
| Peptide sequence | Activity (pmol min−1 µg−1) | Source protein(s) | Ref a |
|---|---|---|---|
| ac‐FR{K‐ac}RW‐am | 19.5 ± 3.6 | Arf‐GAP with dual PH domain‐containing protein 1 (ADAP1) | 3 |
| ac‐SL{K‐ac}FG‐am | 12.1 ± 2.4 | Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHD) | 3, 6 |
| ac‐IS{K‐ac}FD‐am | 6.9 ± 2.0 | AT‐rich interactive domain‐containing protein 1A (ARID1A) | 25 |
| ac‐LT{K‐ac}SP‐am | 5.9 ± 2.8 | Non‐muscle caldesmon (CALD1) | 5 |
| ac‐FA{K‐ac}WR‐am | 4.5 ± 2.1 | Fructose‐bisphosphate aldolase A (ALDOA); Fructose‐bisphosphate aldolase C (ALDOC) | 3, 6 |
| ac‐PV{K‐ac}FI‐am | 3.5 ± 0.7 | Cysteine‐rich protein 2‐binding protein (CSRP2BP) | 3, 6 |
| ac‐YS{K‐ac}GF‐am | 2.8 ± 1.4 | Src substrate cortactin (SRC) | 3, 6 |
| ac‐YQ{K‐ac}WD‐am | 2.5 ± 0.8 | Structural maintenance of chromosomes protein 3 (SMC3) | 3, 6 |
| ac‐AR{K‐ac}ST‐am | 2.2 ± 0.3 | Histone H3.1t (HIST3H3); Histone H3.3 (H3F3A); Histone H3.1 (HIST1H3A) | 3, 52 |
| ac‐FS{K‐ac}AF‐am | 2.2 ± 0.8 | Nucleolar RNA helicase II (DDX21) | 3, 5, 6 |
| ac‐TG{K‐ac}TF‐am | 1.6 ± 0.3 | Actin‐related protein 2/3 complex subunit 2 (ARPC2) | 3, 5 |
| ac‐VI{K‐ac}GF‐am | 1.3 ± 0.1 | Transferrin receptor protein 1 (TFRC) | 4 |
| ac‐LA{K‐ac}HA‐am | 0.9 ± 0.8 | Histone H2B type 1‐K (HIST1H2BK) | 3, 5, 6 |
| ac‐LH{K‐ac}LL‐am | 0.6 ± 0.5 | Nuclear receptor co‐activator 3 (NCOA3); Nucleoprotein TPR (TPR) | 3, 5, 6, 25 |
| ac‐SD{K‐ac}TI‐am | None detected | Tubulin alpha‐3 chain (TUBA3A) | 5 |
a
Reference source that the sequence is acetylated, which usually does not coincide with a report that the sequence is a substrate for KDAC8.