Table 3.

Endpoint Activity for Selected Peptides with Sirt1 and KDAC6

	Activity (pmol min−1 µg−1)
Peptide sequence	Sirt1	KDAC6	KDAC8d	Source protein
ac‐QLS{K‐ac}WP‐am	21.2 ± 2.9a	—b	—b	FOXO3
ac‐DGQMPSD{K‐ac}TIGGGD‐am	—b	1.2 ± 0.2c	—b	TUBA3A
ac‐FR{K‐ac}RW‐am	38 ± 6	16 ± 6	19.5 ± 3.6	ADAP1
ac‐SL{K‐ac}FG‐am	18.6 ± 1.4	4.3 ± 0.8	12.1 ± 2.4	SDHD
ac‐IS{K‐ac}FD‐am	7.3 ± 0.4	7.2 ± 2.2	6.9 ± 2.0	ARID1A

^aPredicted activity calculated from previously reported k _cat and K _M in the sirtuin coupled assay is 50.0 ± 2.5 pmol min⁻¹ µg⁻¹.54

^bNot reported.

^cPreviously reported as a substrate but not characterized with absolute rate.59

^dData from Table 2 repeated for comparison.