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. 2015 Dec 26;25(2):442–455. doi: 10.1002/pro.2833

Figure 5.

Figure 5

mTGase‐catalyzed generation of site specific antibody drug conjugates. (A) Chemical reaction catalyzed by mTGase. (B) Surface representation of anti‐TROP2 IgG1. Extra amino acids “LLQGA” (bold face) at the C‐terminus represent the glutamine tag, with reactive glutamine highlighted in red. Amino acids “LSPG” represent last C‐terminal residues of anti‐TROP2 antibody. (C) Chemical structure of the Linker‐payload utilized to generate the antibody drug conjugate.36 (D) Hydrophobic interaction chromatography (HIC) traces of anti‐TROP2‐AcLys‐vc‐0101 conjugated with wild type S. mobarensis. mTGase (blue) and anti‐TROP2‐AcLys‐vc‐0101 conjugated with mTGase produced and purified from E. coli (pro‐domain mutant Y14A) (red). Antibody anti‐TROP2 prior to conjugation is shown as black lines and conjugation yields [average drug‐antibody ratio (DAR)] are shown in graph titles.