Table 1.
Peptides Modeling a Ser Mutation and a Similar Natural Interruption, Showing Thermal Transition Temperature (T m) and Mean Residue Ellipticity at 225 nm (MRE225) at pH 7.0 and pH 3.0
| Peptide name | Peptide sequencea | pH | T m (°C) | MRE225 (deg cm2 dmol−1) |
|---|---|---|---|---|
| ERSEQ | Ac‐GPO‐GPO‐GPO‐GPO‐GPO‐GPO‐GER‐SEQ‐GPO‐GPO‐GPO‐GPO‐GPO‐GPO‐CONH2 | 7.0 | 46.5 (33.0)b | 4221 |
| 3.0 | 40.8 | 3410 | ||
| DRSER | Ac‐GPO‐GPO‐GPO‐GPO‐GPO‐GPO‐GDR‐SER‐GPO‐GPO‐GPO‐GPO‐GPO‐GPO‐CONH2 | 7.0 | 46.0 | 3539 |
| 3.0 | 41.7 | 3284 |
a
15N‐labeled residues are underlined.
b
Peptide ERSEQ shows two thermal transitions: one major transition with T m = 46.5°C and one minor transition with T m = 33.0°C.