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Representative isothermal titration calorimetry for the binding of sulfanilamide to MtrF. (A) Each peak corresponds to the injection of 10 μL of 40 μM monomeric MtrF in buffer containing 20 mM Tris‐HCl pH 7.5 and 0.03% DDM into the reaction containing 1.0 mM sulfanilamide in the same buffer. (B) Cumulative heat of reaction is displayed as a function of the injection number. The solid line is the least‐square fit to the experimental data, giving a K D of 1.14 ± 0.01 μM.
