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MICs of Sulfamethazine, Sulfadiazine, Sulfathiazole, and Sulfanilamide for Different MtrF Variants Expressed in E. coli BL21(DE3)ΔabgTΔpabA
| Gene in BL21(DE3)ΔabgTΔpabA | Sulfamethazine (μg/mL) | Sulfadiazine (μg/mL) | Sulfathiazole (μg/mL) | Sulfanilamide (μg/mL) |
|---|---|---|---|---|
| Empty vector | 62.5 | 31.25 | 62.5 | 500 |
| mtrF (wild‐type) | 2000 | >250 | >500 | 4000 |
| mtrF (D193A) | 1000 | 31.25 | 62.5 | 2000 |
| mtrF (W420A) | 125 | 31.25 | 62.5 | 2000 |
| mtrF (P438A) | 62.5 | 31.25 | 62.5 | 1000 |
| mtrF (D449A) | 62.5 | 31.25 | 62.5 | 1000 |
