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Editing activity of EMD fragments of EcPheRS against Tyr‐tRNAPhe: (A) B1–B5 and (B) B1–B4. Reactions were performed with 1.2 µM Tyr‐tRNAPhe prepared from in vitro transcribed E. coli tRNAPhe, with the addition of 4 µM B1–B5, 4 µM B1–B4, and 1.2 µM chimera or in the absence of enzyme.
