Table 1.
Target peptides employed for the quantification of hemagglutinin (HA) and neuraminidase (NA). Underlined amino acids correspond to 13C- and 15N-labeled amino acids. N9, neuraminidase of H7N9 strains; H7, hemagglutinin of H7N7 strains; N7, neuraminidase of H7N7 strains.
| Target Peptide | influenza protein | precursor ion m/z | fragment ion (quantification) | fragment ion (confirmation) | fragment ion (confirmation) | collision energy (eV) |
|---|---|---|---|---|---|---|
| IGESSDVLVTR | N9 | 588.3 (+2) | 702.4 (y6) | 789.4 (y7) | 876.5 (y8) | 23 |
| IGESSDVLVTR | N9 | 591.3 (+2) | 708.4 (y6) | 795.5 (y7) | 882.5 (y8) | 23 |
| FYALSQGTTIR | N9 | 628.8 (+2) | 762.4 (y7) | 875.5 (y8) | 946.5 (y9) | 24 |
| FYALSQGTTIR | N9 | 632.3 (+2) | 769.4 (y7) | 882.5 (y8) | 953.5 (y9) | 24 |
| VPNALTDDR | N9 | 500.8 (+2) | 619.3 (y5) | 690.3 (y6) | 804.4 (y7) | 20 |
| VPNALTDDR | N9 | 504.3 (+2) | 626.3 (y5) | 697.3 (y6) | 811.4 (y7) | 20 |
| FGESEQIIVTR | N7 | 639.8(+2) | 729.4 (y6) | 858.5 (y7) | 945.5 (y8) | 23 |
| FGESEQIIVTR | N7 | 642.8 (+2) | 735.5 (y6) | 864.5 (y7) | 951.6 (y8) | 23 |
| IPNAGTDPNSR | N7 | 571.3 (+2) | 473.2 (y4) | 689.3 (y6) | 931.4 (y9) | 25 |
| IPNAGTDPNSR | N7 | 574.3 (+2) | 479.3 (y4) | 695.3 (y6) | 937.4 (y9) | 25 |
| SGYETFR | N2 | 430.2(+2) | 715.3 (y5) | 772.4 (y6) | 552.3 (y4) | 18 |
| SGYETFR | N2 | 435.2 (+2) | 725.4 (y5) | 782.4 (y6) | 562.3(y4) | 18 |
| VNTLTER | H7 | 416.7(+2) | 518.3 (y4) | 619.3 (y5) | 733.4 (y6) | 17 |
| VNTLTER | H7 | 420.2 (+2) | 525.3 (y4) | 626.4 (y5) | 740.4 (y6) | 17 |
| FVNEEALR | H7 | 489.3 (+2) | 617.3 (y5) | 731.4 (y6) | 830.4 (y7) | 20 |
| FVNEEALR | H7 | 492.8 (+2) | 624.3 (y5) | 738.4 (y6) | 837.5 (y7) | 20 |
| IQIDPVK | H7 | 406.7(+2) | 343.2 (y3) | 458.3 (y4) | 571.3 (y5) | 17 |
| IQIDPVK | H7 | 409.7 (+2) | 349.2 (y3) | 464.3 (y4) | 577.4 (y5) | 17 |
| STQSAIDQITGK | H7 | 624.8(+2) | 661.3 (y6) | 774.4 (y7) | 845.5 (y8) | 25 |
| STQSAIDQITGK | H7 | 628.3 (+2) | 668.4 (y6) | 781.5 (y7) | 852.5 (y8) | 25 |