Table 2. Cline parameters and main genetic estimates for 13 P. glauca, P. engelmannii and hybrid populations based on 10 microsatellite loci.

Loci ID	Center (km)	Slope	Width (km)	Ht	Mean He	Mean Ho	Fis
SSR01	1999	(−) 0.25	400.95	0.503	0.489	0.459	−0.018
SSR02	0.01	(−) 0.34	289.98	0.828	0.807	0.754	0.06
SSR03	0.01	(−) 1.74	57.35	0.718	0.688	0.667	0.007
SSR04	35	(+) 1.67	59.82	0.506	0.454	0.307	0.206
SSR05	0.02	(−) 1.97	50.74	0.74	0.69	0.73	−0.02
SSR06	441	(−) 1.81	55.27	0.628	0.582	0.445	0.095
SSR07	0.08	(−) 1.99	50.09	0.156	0.145	0.137	0.03
SSR08	0.03	(−) 1.79	55.75	0.212	0.207	0.176	0.072
SSR09	1999	(−) 0.63	158.03	0.726	0.647	0.354	0.173
SSR10	622	(−) 1.95	51.14	0.818	0.789	0.61	0.087
Mean	509.61	1.41	122.9	0.583	0.55	0.464	0.07

The mean expected heterozygosity (Mean H_e), mean observed heterozygosity (Mean H_o) and inbreeding coefficient (F_is) were averaged across all populations for each loci. Loci ID can be found in Supplementary Table S1 in Supplementary Information. H_o, H_e and F_is estimates within population by locus can be found in Supplementary Table S3 in Supplementary Information.