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Variation in cortisol concentrations of successive clutches extruded from female threespine stickleback (Gasterosteus aculeatus) after prolonged (24 h; open circles) or short (<8 h; closed circles) post-ovulatory egg retention. A two-way repeated measures ANOVA indicated nonsignificant effects of population or population by treatment. Hormone concentration of clutches differed between short and prolonged treatment in the limnetic population but not in the benthic (contrast: P<0.001).
