Figure 1.

Comparison of brain slice perfusion methods. (A) Forced convection intra-culture perfusion. The flow of equilibrated medium is forced to pass through the mass of cells and through the full culture thickness. (B) Superfusion and capillary wicking of superfused medium by the tissue slice. The flow of equilibrated medium is not forced to pass through the mass of cells and through the full culture thickness. The resistance to fluid flow through the culture thickness is much higher than the resistance to fluid flow in a channel below the tissue. Flow passes through the channel and the tissue slice wicks the medium from the channel by capillarity.