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. 2014 Dec 24;114(3):356–365. doi: 10.1038/hdy.2014.111

Figure 1.

Figure 1

Schematic representation of T. dubius and T. porrifolius rDNA units. (a, b) The structures of T. porrifolius and T. dubius IGS regions, respectively, occurring between the 18S-5.8S-26S genic regions (c) are shown. The IGS includes repetitive elements (grey boxes) and the gene (P) and spacer (S) promoters. Double head arrow in (a, b) indicates a region used for bisulfite sequencing; positions of T. dubius-specific primers used for IGS amplification are marked with arrows. (d) Scheme of the ITS1 flanked by the 18S and 5.8S genes of T. dubius and T. porrifolius. The position of the probe used to detect restriction fragment length polymorphisms with BstNI is indicated by a line, along with the lengths of the restriction fragments. The arrows indicate primer positions: 18S_for, ITS_109, 5.8S_rev, por_R776, 454 MID_18S and MID_5.8S.