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. 2014 Dec 24;114(3):356–365. doi: 10.1038/hdy.2014.111

Figure 2.

Figure 2

Genetic characterisation of rDNA in T. mirus plants 33A and 33B. (a) Southern blot hybridisation of genomic and plasmid DNA using the ITS1 probe. Left part shows a series dilution of cloned ITS1 fragments for rDNA unit copy number estimations, and right, probe hybridisation against BstNI-restricted DNAs from T. mirus and its parents, T. porrifolius and T. dubius. Units are identified as being of T. porrifolius (P-units) or T. dubius (D-units) origin. The P/D ratio is shown. (b) Detection of long and short IGS variants in T. mirus D-genome using T. dubius-specific PCR primers. (c) Detection of long and short IGS variants in T. mirus D-genome by Southern blot analysis with the SspI and BstYI restriction enzymes. Sample ‘31' is an individual from the Rosalia population.