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. 2014 Nov 5;114(3):309–317. doi: 10.1038/hdy.2014.101

Figure 3.

Figure 3

Karyotyping using FISH and GISH reprobing. (a, c, e, g and i) FISH probes were as follows: centromeric TPRMBO (lilac), subtelomeric TGP7 (red), interstitial TTR3 (green) and 18 S rDNA (yellow/orange). (b, d, f, h and j) GISH reprobing of the same chromosome preparations using total genomic DNA of the parents, T. dubius (green) and T. porrifolius (red). Diamond symbols are placed below aneuploid chromosomes. Arrows indicate the position of translocation breakpoints revealed by GISH. Arrowheads indicate missing FISH markers that did coincide with a translocation detected with GISH. (a and b) 2866-11-1, (c and d) 2866-15-1, (e and f) 2869-9-1, (g and h) 2869-10-1 and (i and j) 2876-10-1. Scale bar, 5 μm.