FISH using the U1 snDNA as a probe in species of Proscopiidae (a and b), Pyrgomorphidae (c and d) and Ommexechidae (e), shown in metaphase I (a, c and e), metaphase II (b) and diplotene (d) cells. The chromosomes carrying U1 snDNA signals and the X chromosome are indicated. c centromere location. The inset in e corresponds to pair 3 from a metaphase II cell, showing the location of the two U1 snDNA clusters (centromeric and proximal). Bar=5 μm.