Chromosomal location of U1 snDNA in nine Romaleidae species, shown in metaphase I (a, b, d, f, h and i), metaphase II (c) and diplotene (e and g) cells. The chromosomes carrying U1 snDNA signals and the X chromosome are indicated. c centromere location. The inset in a corresponds to the eighth chromosome from an anaphase I cell, showing U1 clusters at centromeric and proximal/proximal locations. Bar=5 μm.