Table 1. Summary of genetic markers screened and mapped in the Senecio aethnensis × S. chrysanthemifolius F2 genetic mapping family.
| Marker type | No. of screened | No. of developed | No. of genotyped | No. of mapped | Unlinked | Problematic |
|---|---|---|---|---|---|---|
| Codominant markers | ||||||
| EST indel | 45 | 15 | 10 | 8 | EC77, EC1687 | |
| EST SSR | 216 | 48 | 33 | 31 | ES91 | ES19 |
| Indel | 7 | 4 | 3 | 3 | ||
| SSR | 72 | 9 | 8 | 8 | ||
| Total | 340 | 76 | 54 | 50 | 1 | 3 |
| Dominant markers | ||||||
| E1M3, CAAC/ACAG | — | — | 19 | 15 | 179, 219 | |
| E1M5, CAAC/ACTA | — | — | 15 | 10 | 168, 213 | 88, 160, 204 |
| E1M7, CAAC/ACTG | — | — | 13 | 12 | 275 | |
| E4M7, CACT/ACTG | — | — | 9 | 8 | 113 | |
| E5M3, CACC/ACAG | — | — | 8 | 7 | 302 | |
| E5M6, CACC/ACTC | — | — | 9 | 9 | ||
| E8M5, CAGG/ACTA | — | — | 11 | 9 | 114 | 196 |
| E8M7, CAGG/ACTG | — | — | 7 | 7 | ||
| Total | — | — | 91 | 77 | 8 | 6 |
Abbreviations: EST, expressed sequence tag; SSR, simple sequence repeat.
Marker types are divided into codominant or dominant markers. Codominant markers are further divided into markers derived from ESTs, SSRs or insertion-deletions (indel). Dominant markers are amplified fragment length polymorphisms (AFLPs). Each AFLP primer combination is shown as the primer names used in this study followed by the three selective bases for the EcoRI and MseI primers, respectively (Supplementary Methods). No. of screened is number of codominant marker primer pairs tested. No. of developed is number of codominant markers that could be scored in Senecio. No. of genotyped is number of markers showing polymorphism in the F2AC mapping family. No. of mapped is number of markers that were included in the final genetic map. Unlinked names markers that were unlinked at a >4 logarithm of odds score or>20 cM linkage threshold limits for mapping. Problematic refers to markers that caused problems with linkage group marker order or were present in multiple linkage groups. Dominant marker names are approximate fragment base-pair lengths used to label AFLP loci.