Figure 3.

RNA-seq expression profiling of the bank vole α-globin genes. (a) The mean RPKM of each HBA paralogue with error bars representing s.e.m. (b) Fold difference in transcript abundance between two alleles for each HBA paralogue (that is, AI). In the first three columns, the error bar represents s.e.m. The rightmost column (hatched) shows the result for an individual heterozygous (Het) for the allele containing a premature stop codon in exon 2 (PTC1). (c) RNA-seq depth-of-coverage profiles of an allele with an uninterrupted open reading frame and of the PTC1-containing allele from the heterozygote.