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. 2016 Mar;101(3):286–296. doi: 10.3324/haematol.2015.133637

Figure 1.

Figure 1.

Immunophenotyping of peripheral blood (PB) CD34+ cells in erythroid culture. (A) Surface expression of CD34, CD45, CD38, IL-3R (CD123), CD45RA and CD90 on PB CD34+ cells on the day of isolation. All populations were gated first on Propidium iodide incorporation and scatter properties. Cells were gated for CD34+ and CD45lo (left panel) prior to analysis of CD38 and CD90. IL-3R and CD45RA were analyzed on the total live population. Gates were set with isotype controls. (B) Surface expression of IL-3R and Flt3 on CD34+ cells cultured in EM for four days. Stained cells in blue and isotype controls in orange. Expression of both markers increases in the total population during the first three days of culture and then decreases. (C) Surface expression of CD36 over four days of culture demonstrating that CD36− and CD36+ cells comprise distinct populations. Stained cells in blue and isotype controls in orange.