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. 2016 Mar;101(3):371–381. doi: 10.3324/haematol.2015.134155

Figure 3.

Figure 3.

Different NK cell subsets identified by NKG2C/CD57 expression: progressive expansion of memory-like NKG2C+CD57+ NK cells in patients reactivating HCMV after HSCT. PBNK cells from the various patients were analyzed for the expression of NKG2C and CD57 at 1, 3, 6 and 12 months after HSCT. After gating on CD56+CD3−CD19− lymphocytes, CD56dim NK cells were evaluated. In (A) the size of the different subsets identified by NKG2C and CD57 on CD56dim NK cells (i.e. NKG2C+CD57−, NKG2C+CD57+, NKG2C−CD57− and NKG2C−CD57+, indicated for brevity as 2C and 57) in patients experiencing (empty circles, n=13) or not (filled black squares, n=14) HCMV reactivation after transplantation are shown from month 1 to month 12 after HSCT. 95% CI for the mean and statistical significance are indicated (*P<0.05; ** P<0.01; *** P<0.001). In (B), reciprocal expression of NKG2C and CD57 on CD56dim NK cells from two representative patients, one reactivating (upper panels), and the other non-reactivating HCMV (lower panels), is shown at different time intervals after transplantation in comparison to NK cells isolated from their respective HSC donors (left panels). The percentages of positive cells are indicated in each quadrant. In (C) reciprocal expression of NKG2C and CD57 on CD56dim NK cells from three HDs (one HCMV−, left panel; two HCMV+, middle and right panels) are shown. The percentages of positive cells are indicated in each quadrant.