Figure 1.

Expression of E-Cadherin, β-Catenin and Calpain according to the mode of tumour progression. Western blotting analysis showing representative expression of E-Cadherin (E-Cad-85 and E-Cad-23), β-Catenin and Calpain in the different types of tumour dissemination. Protein lysates from the breast cancer cell line MCF7 were used as positive controls for E-Cadherin, from the ovarian cell line SKOV3 for β-Catenin and from the cervical cancer cell line HELA for Calpain. Equal amounts of protein lysate (20 μg) were loaded per well. As a loading control, GAPDH protein expression was detected.