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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Autoimmunity. 2015 Aug 31;48(8):513–523. doi: 10.3109/08916934.2015.1077233

Figure 1.

Figure 1

Layout and content of silicon-based U1-70K peptide microarrays. (A) Peptides representing sequences from amino acids 110–170 of the RNA-binding domain of the human U1-70K protein were synthesized using maskless photolithography on the surface of a silicon wafer. (B) The microarray consists of overlapping 3- to 10-mer peptides in triplicate, tiled with a one amino acid offset across the region (upper), and duplicate 21 × 21 feature blocks representing every possible 1- to 21-mer within the sequence ranges shown below (lower). (C) An expanded view of a block featuring peptides derived from amino acid sequence 110–130 is shown. The peptide sequences of features from the bottom row and right-most column are shown for illustration.