Figure 4.

Mitochondrial fragmentation and OPA1 loss in IKKα- and IKKαβ-deficient MEFs. (a) OPA1 profile in WT, IKKαβ^−/−, IKKα^−/−, and IKKβ^−/− MEFs. Immunoblot was probed with specific OPA1 antibodies. Hsp60 was used as loading control to normalize protein content. (b) Results correspond to the mean of three experiments. *P<0.05 compared with WT and IKKβ^−/− MEF cells. (c) WT and in IKKαβ^−/− and IKKα^−/− cells were stained with specific antibodies directed against cytochrome c (Cyt c, in green) and HSP60 (in red). (d) The histograms show the percentage of cells displaying mitochondrial fragmentation (% fragmented mitochondria) and the release and disappearance of cytochrome c (% diffuse Cyt c). For each condition, 150 cells were analyzed. Mean±S.D. of three independent experiments; *P<0.05