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. 2015 Jun 5;23(1):41–51. doi: 10.1038/cdd.2015.61

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Loss of Bim delays apoptosis during involution. (a) Representative examples of immunofluorescence-based TUNEL analysis performed on mammary gland #4 sections from tissue isolated on day 0 or day 3 of involution from wt, Bmf^−/− or Bim^−/− mice. (b) Quantification of samples shown in a. Bars represent means ±S.E. of n=3 mice per day and genotype analysed. (c) Representative examples of immunohistochemistry-based analysis of caspase-3 activation performed on mammary gland #4 sections from tissue isolated on day 0 or day 3 of involution from wt, Bim^−/− or Bmf^−/− mice. (d) Quantification of samples shown in c. Bars represent means ±S.E.M. of n=3 mice per day and genotype analysed. *indicates a statistically significant difference between wt and Bim-deficient mice, as assessed by unpaired Student's t-test (P<0.05)