Figure 6.

PIKE-A mediates cell proliferation via suppressing AMPK activity. (a) AMPKα −/− MEF was transfected with wt or constitutive active mutant of AMPKα, followed by PIKE-A adenovirus infection. Cell proliferation was determined by cell number of each cell line after 3 days seeding (n=3). The cell lysates were analyzed by immunoblotting with the indicated antibodies. The error bars represent mean values±S.D. from three replicates of each sample (**P<0.01). (b) Infection of LN229 cells with control and PIKE-A adenovirus, in the presence or absence of AICAR (0.2 mM). Cell proliferation was examined by cell number of each cell line after 3 days seeding (**P<0.01, n=3). The cell lysates were analyzed by immunoblotting with the indicated antibodies. (c) LN229 cells were infected with shRNA of PIKE-A adenovirus, followed by treatment with compound C (0.2 μM). Cell proliferation was examined by cell number of each cell line after 3 days seeding (**P<0.01, n=3). The cell lysates were analyzed by immunoblotting with the indicated antibodies