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. 2015 Jun 12;23(1):132–145. doi: 10.1038/cdd.2015.78

Figure 1.

Figure 1

Expression of the miR-200 s is downregulated in CAFs. (a) Heat map illustrating hierarchical clustering of altered miR-200 family members in CAFs and paired NFs. (b) The expression of miR-200 s identified by miRNA array was confirmed using qRT-PCR in paired NFs and CAFs from three breast cancer patients. U6 was used as an internal control. The relative expression levels are shown as the fold change of CAFs compared with NFs (n=3). (c) The expression of the miR-200 family was evaluated by qRT-PCR in 20 paired NF and CAF samples that were freshly isolated from breast carcinoma tissues. U6 was used as an internal control. The data are shown as the normalized fold change of CAFs compared with NFs (n=3). (d) The expression of the miR-200 family was examined by qRT-PCR in NFs co-cultured with four different breast cancer cell lines (BT474, MCF-7, MDA-MB-453 and MDA-MB-468) for 15 or 30 days. U6 was used as an internal control. The data are shown as the normalized fold change of co-cultured NFs compared to NFs alone (n=3)