Rac1 is expressed in the periimplantation mouse uterus in a spatiotemporal manner. (a) In situ hybridization of Rac1 mRNA in D1-8 uteri. The pink site indicates Rac1's location. Positive signals were not detected in the uterus labeled with the Sense probe. myo, myometrium; M, mesometrium; AM, anti-mesometrium. The white arrowhead indicates the embryo. Scale bars, 200 μm. (b) Immunofluorescence of Rac1 protein in D1-8 uteri. Red signals represent Rac1 protein and the nucleus is marked with DAPI. The white arrowhead indicates the embryo. le, luminal epithelium; s, stroma; M, mesometrium; AM, anti-mesometrium. Scale bars, 100 μm. (c) Immunohistochemistry of active Rac1 in D1-8 uteri. The arrowhead indicates the embryo. le, luminal epithelium; s, stroma. Scale bars, 100 μm