Figure 7.

Rac1-deficient luminal epithelium surrounding the implanting embryo fails to undergo apoptotic death at postimplantation. (a) HE and Cox2 staining in Rac1^f/f and Rac1^d/d D5 implantation sites. Scale bars, 100 μm. (b) Gross histology of Rac1^f/f and Rac1^d/d implantation sites on D6. M, mesometrium; AM, anti-mesometrium. The black arrowheads indicate embryos. Scale bars, 100 μm. (c) A persisted luminal epithelial layer within the Rac1^d/d implantation chambers on D6 visualized by immunofluorescence staining of E-cadherin and β-catenin. The white arrowheads indicate embryos. Scale bars, 75 μm. (d) TUNEL assay and cleaved Caspase 3 staining in Rac1^f/f and Rac1^d/d day 6 implantation sites. The black arrowheads indicate embryos. The blue arrowhead indicates apoptotic luminal epithelial cells. Scale bars, 100 μm