Table 2.

SCAD missense mutations analysis with prediction software tools.

Variant	MutPred Probability of deleterious mutation	MutPred2 Probability of deleterious mutation	Molecular mechanism disrupted hypotheses (P⁎< 0.05)
c.700C > T (p.Arg234Trp)	0.581	0.838	Loss of disorder
c.814C > T (p.Arg272Cys)	0.966	0.917	Loss of methylation at Arg272 Gain of palmitoylation at R272
c.842G > C (p.Gly281Ala)	0.979	0.938	–
c.869C > G (p.Ala290Gly)	0.761	0.763	Loss of stability
c.1054G > A (p.Ala352Thr)	0.788	0.876	–
c.1130C > T (p.Pro377Leu)	0.754	0.836	Gain of stability Loss of disorder
c.1157G > A (p.Arg386His)	0.905	0.917	Loss of stability

^⁎P is the P-value that certain structural and functional properties are impacted.