Figure 8.

Knockdown of TDP-43 enhances CVB3 replication. HeLa cells were transfected with an siRNA targeting TDP-43 (siTDP-43) or a scramble siRNA (siCon) for 48 h followed by CVB3 infection for 16 h at an multiplicities of infection (MOI) of 1. (a) Western blotting was conducted to examine protein expression of TDP-43 and β-actin (loading control). Densitometric analysis was performed as in Figure 1. * indicates the N-terminal cleavage fragment of TDP-43. (b) Plaque assay was carried out to assess viral titers in the supernatant. Viral titers were expressed as plaque formation units (pfu) per ml and presented as mean±S.D. (n=3), *P<0.05. (c) Localization of TDP-43 and dsRNA in the cytoplasm of CVB3-infected cells. HeLa cells were transfected with HA-TDP-43 for 24 h, followed by CVB3 infection for 3 h. Immunostaining was carried out for the detection of TDP-43 using anti-HA antibody (green) and dsRNA using anti-dsRNA antibody (red). Cell nuclei were counterstained with DAPI (blue). Pearson's Correlation Coefficient (PCC) was calculated using Volocity software Version 5.2.1 over at least three images