Figure 5.

Inhibiting fibril growth attenuates α-syn-induced toxicity in hippocampal primary neurons. Hippocampal primary culture were plated in 96-well plates and treated for 6 days with Tris buffer (negative control) or (a) the indicated α-syn species in the presence of DMSO (negative control) or Tolcapone (aggregation inhibitor), (b) with β-syn monomers, a mixture of β-syn monomers and sonicated α-syn PFFs, sonicated Tau PFFs or a mixture composed of α-syn monomers and sonicated Tau PFFs. For each condition cells were stained with SG. Cell death level is expressed as the percentage of cells with compromised cell membrane (SG-positive cells) to the total cell number analyzed by Tecan infinite M200 Pro plate reader (487 nm/519 nm). Shown are the means of three independent experiments performed in triplicate for each condition (bars are means±S.D.). One-way ANOVA test followed by Tukey–Kramer post-hoc test were performed, **P<0.001 for α-syn mixture±Tolcapone (a); *P<0.01, ***P<0.0001. Tris versus α-syn, β-syn or Tau-treated conditions (b)