Table 4.
Comparison of B cell and T cell area measurement by the area quantification and positive pixel count analysis
| Tissues | Area quantification (% positive area/total area) |
Positive pixel count analysis (% positive pixels/total pixels) |
||||
|---|---|---|---|---|---|---|
| B cell area (CD79a) | T cell area (CD3) | Ratio of B cell to T cell area | B cell area (CD79a) | T cell area (CD3) | Ratio of B cell to T cell area | |
| Ileocecal plaque | 53 ± 5 | 24 ± 7 | 2.1 | 44 ± 9 | 25 ± 6 | 1.9 |
| Cecal tonsil | 55 ± 3 | 14 ± 2 | 3.9 | 43 ± 4 | 17 ± 3 | 2.6 |
| Peyer's patch | 44 ± 18 | 22 ± 10 | 2.2 | 42 ± 4 | 22 ± 4 | 2 |
| Appendix | 70 ± 5 | 10 ± 1 | 7.3 | 52 ± 9 | 9 ± 1 | 5.6 |