Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Dec 4;26(1):119–130. doi: 10.1038/cr.2015.143

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

This license allows readers to copy, distribute and transmit the Contribution as long as it attributed back to the author. Readers are permitted to alter, transform or build upon the Contribution as long as the resulting work is then distributed under this is a similar license. Readers are not permitted to use the Contribution for commercial purposes. Please read the full license for further details at - http://creativecommons.org/licenses/by-nc-sa/4.0/

PMC Copyright notice

The fate of Kit⁺ cells in heart homeostasis. (A) Kit-CreER mice were crossed with Rosa26-RFP mice for lineage tracing. (B) A schematic of tamoxifen induction and analysis. (C) Whole mount bright field and fluorescence view of Kit-CreER;Rosa26-RFP hearts with or without tamoxifen (Tam or No Tam). Arrow indicates RFP⁺ cardiomyocyte. (D-F) Immunostaining for RFP, TNNI3 and PECAM on Kit-CreER;Rosa26-RFP heart sections. (G, H) Z-stack confocal images including XZ scanned sections showing RFP⁺TNNI3⁺ cells (arrows, G) and RFP⁺PECAM⁺ cells (arrowheads, H). (I) Image of cells dissociated from hearts of Kit-CreER;Rosa26-RFP mice. Arrow indicates a rare RFP⁺ cardiomyocyte. (J) Quantification of RFP⁺ cardiomyocytes by flow cytometry. Events in images are gated on live and lineage-negative cardiomyocytes. n = 4. Scale bars represent 1 mm in C, 100 μm in D-I.