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. 2015 Dec 8;26(1):66–82. doi: 10.1038/cr.2015.145

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Copyright © 2016 Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences

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Levels of siRNAs, P4 (Pol IV-dependent) RNAs and DNA methylation in dcl mutants. (A) Northern blot analysis of miRNA, siRNAs and P4 RNAs at the indicated loci. The dcl1/2/3/4 mutant contained the dcl1-201 allele. (B) Boxplots of DNA methylation levels at nrpd1 hypo-mC-DMRs in the various genotypes. Col-0-A, dcl2/3/4-A and dcl1/2/3/4-A are adult (5-week-old) plants of the Col-0, dcl2/3/4 and dcl1/2/3/4 (left, dcl1-201; right, dcl1-202) genotypes, respectively. Except for the dcl1/2/3/4 mutants, each genotype had two replicates. (C) IGV genome browser screenshots showing the DNA methylation levels at the indicated loci. For dcl1/2/3/4, the upper row had the dcl1-201 allele and the lower one had the dcl1-202 allele.